ABSTRACT
Objective:Meta analysis was used to evaluate the diagnostic value of Septin-9 methylation in colorectal cancer.Methods:Research on the diagnostic value of Septin-9 methylation in WanFang, CNKI, Weip, Pubmed and Embase. The release time is from inception to January, 2020. After data extraction, QUADAS-2 standard was used to evaluate the quality of the included literature. Then the extracted data were preliminarily combined to determine the threshold effect, heterogeneity and diagnostic efficacy, and the source of heterogeneity and subgroup analysis were further discussed through sensitivity analysis. Finally, funnel plot was used to evaluate whether publication bias existed.Results:A total of 17 articles were included in the study, including 1 668 cases in the case group and 2 407 cases in the control group.The combined sensitivity: 0.74 (95% CI: 0.72-0.76), specificity: 0.81 (95% CI: 0.79-0.83), positive likelihood ratio: 6.89 (95% CI: 3.39-12.19), negative likelihood ratio: 0.30 (95% CI: 0.23-0.40), diagnostic ratio: 23.43 (95% CI: 10.25-53.53), area under curve (AUC) of SROC (summary ROC): 0.821. Subgroup analysis showed that Septin-9 methylation detection was of high diagnostic value in the diagnosis of colorectal cancer by tissue samples and nested methylation specific PCR (DOR=63.355, 56.567), while the diagnostic value after surgical intervention is low (DOR=14.245). Conclusions:The methylation detection of septin-9 gene in Chinese population is of high value in the diagnosis of colorectal cancer and is expected to become a diagnostic marker of colorectal cancer.
ABSTRACT
Objective:Meta analysis was used to evaluate the diagnostic value of miRNA-25 in different malignant tumors in Chinese population.Methods:The research on the diagnostic value of miRNA-25 in China national knowledge internet (CKNI), VIP, Wanfang, PubMed and EMBase was searched. The publication time was from the establishment of database to October 2019. After the data was extracted, the Meta.DiSc1.4 software was used for preliminary statistical analysis. The Review Manager 5.3's QUADAS-2 tool was used for quality control of the incoming literature. The STATA 15.0 software was used to analyze whether there was publication bias.Results:A total of 10 articles were included in the study, including 792 cases in the case group and 715 cases in the control group. The combined sensitivity, combined specificity, combined specificity, combined positive likehood ratio(PLR), negative likehood ratio (NLR) were 0.71(95% CI: 0.69-0.74), 0.84(95% CI: 0.81-0.86), 4.29(95% CI: 3.39-5.42), 0.35(95% CI: 0.31-0.40), respectively. Diagnostic odds rate (DOR) was 13.09(95% CI: 9.64-17.78), and the area under curve (AUC) was 0.80(95% CI: 0.77-0.84). Subgroup analysis showed that the diagnostic value of miRNA-25 for different malignant tumors suggested that the lung cancer group had the highest diagnostic value and specificity (DOR=16.418, SPE=0.863). Funnel plot indicated that no significant publication bias was found ( P=0.21). Conclusions:miRNA-25 is highly sensitive and specific to different malignancies, especially lung cancer, and can be used as a potential screening biomarker to assist diagnosis.
ABSTRACT
Objective To observe the effect of Chuanxiongqin injection combined with nimodipine on primary open angle glaucoma.Methods 112 patients with primary open angle glaucoma in ophthalmology department were grouped two groups,each with 56 cases.Two groups were treated with nimodipine and observation group was treated another with Chuanxiongqin injection to observe effect.Results After treatment,retinal light sensitivity,visual field defect,blood flow,blood flow velocity,erythrocyte migration rate,PSV,EDV and RI in central retinal artery of observation group were better than control group(P<0.05).There was no significant difference in adverse reactions between groups.Conclusion Chuanxiongqin injection can improve blood flow status of central retinal artery and optic papillary laminar, retinal light sensitivity and visual field defect for patients with primary open angle glaucoma.